Quantitation of monoclonal antibody by capture ELISA based on initial enzyme activity rate

We developed a noncompetitive two-site sandwich ELISA to quantitate monoclonal antibodies in culture supernatant. This assay measures the initial enzyme activity rate during the first minute of the reaction, which ensures linear velocity relative to time and a progress curve slope proportional to an...

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Detalles Bibliográficos
Autores principales: Domínguez, Mercedes, Moreno, Inmaculada, Toraño, Alfredo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2019
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7094287/
https://www.ncbi.nlm.nih.gov/pubmed/31404550
http://dx.doi.org/10.1016/j.jim.2019.112645

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7094287/
https://www.ncbi.nlm.nih.gov/pubmed/31404550
http://dx.doi.org/10.1016/j.jim.2019.112645

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