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Quantitation of monoclonal antibody by capture ELISA based on initial enzyme activity rate
We developed a noncompetitive two-site sandwich ELISA to quantitate monoclonal antibodies in culture supernatant. This assay measures the initial enzyme activity rate during the first minute of the reaction, which ensures linear velocity relative to time and a progress curve slope proportional to an...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier B.V.
2019
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7094287/ https://www.ncbi.nlm.nih.gov/pubmed/31404550 http://dx.doi.org/10.1016/j.jim.2019.112645 |