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An improved nonchromatographic method for the purification of recombinant proteins using elastin-like polypeptide-tagged proteases
Proteins fused to the elastin-like polypeptide (ELP) tag can be selectively separated from crude cell extract without chromatography. To avoid the interference of the ELP tag on properties of the target protein, it is necessary to remove the ELP tag from target protein by protease digestion. Therefo...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier Inc.
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7094418/ https://www.ncbi.nlm.nih.gov/pubmed/21600185 http://dx.doi.org/10.1016/j.ab.2011.04.034 |