An improved nonchromatographic method for the purification of recombinant proteins using elastin-like polypeptide-tagged proteases

Proteins fused to the elastin-like polypeptide (ELP) tag can be selectively separated from crude cell extract without chromatography. To avoid the interference of the ELP tag on properties of the target protein, it is necessary to remove the ELP tag from target protein by protease digestion. Therefo...

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Detalles Bibliográficos
Autores principales: Lan, Dongming, Huang, Guangrui, Shao, Hongwei, Zhang, Lichun, Ma, Lixin, Chen, Shangwu, Xu, Anlong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Inc. 2011
Materias:
Notes & Tips
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7094418/
https://www.ncbi.nlm.nih.gov/pubmed/21600185
http://dx.doi.org/10.1016/j.ab.2011.04.034
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author Lan, Dongming
Huang, Guangrui
Shao, Hongwei
Zhang, Lichun
Ma, Lixin
Chen, Shangwu
Xu, Anlong
author_facet Lan, Dongming
Huang, Guangrui
Shao, Hongwei
Zhang, Lichun
Ma, Lixin
Chen, Shangwu
Xu, Anlong
author_sort Lan, Dongming
collection PubMed
description Proteins fused to the elastin-like polypeptide (ELP) tag can be selectively separated from crude cell extract without chromatography. To avoid the interference of the ELP tag on properties of the target protein, it is necessary to remove the ELP tag from target protein by protease digestion. Therefore, an additional chromatographic purification step is required to remove the proteases, and this is time- and labor-consuming. Here we demonstrate the utility of the ELP-tagged proteases for cleavage of ELP fusion proteins, allowing one-step removal of the cleaved ELP tag and ELP-tagged proteases without chromatography.
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spelling pubmed-70944182020-03-25 An improved nonchromatographic method for the purification of recombinant proteins using elastin-like polypeptide-tagged proteases Lan, Dongming Huang, Guangrui Shao, Hongwei Zhang, Lichun Ma, Lixin Chen, Shangwu Xu, Anlong Anal Biochem Notes & Tips Proteins fused to the elastin-like polypeptide (ELP) tag can be selectively separated from crude cell extract without chromatography. To avoid the interference of the ELP tag on properties of the target protein, it is necessary to remove the ELP tag from target protein by protease digestion. Therefore, an additional chromatographic purification step is required to remove the proteases, and this is time- and labor-consuming. Here we demonstrate the utility of the ELP-tagged proteases for cleavage of ELP fusion proteins, allowing one-step removal of the cleaved ELP tag and ELP-tagged proteases without chromatography. Elsevier Inc. 2011-08-15 2011-04-27 /pmc/articles/PMC7094418/ /pubmed/21600185 http://dx.doi.org/10.1016/j.ab.2011.04.034 Text en Copyright © 2011 Elsevier Inc. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Notes & Tips
Lan, Dongming
Huang, Guangrui
Shao, Hongwei
Zhang, Lichun
Ma, Lixin
Chen, Shangwu
Xu, Anlong
An improved nonchromatographic method for the purification of recombinant proteins using elastin-like polypeptide-tagged proteases
title An improved nonchromatographic method for the purification of recombinant proteins using elastin-like polypeptide-tagged proteases
title_full An improved nonchromatographic method for the purification of recombinant proteins using elastin-like polypeptide-tagged proteases
title_fullStr An improved nonchromatographic method for the purification of recombinant proteins using elastin-like polypeptide-tagged proteases
title_full_unstemmed An improved nonchromatographic method for the purification of recombinant proteins using elastin-like polypeptide-tagged proteases
title_short An improved nonchromatographic method for the purification of recombinant proteins using elastin-like polypeptide-tagged proteases
title_sort improved nonchromatographic method for the purification of recombinant proteins using elastin-like polypeptide-tagged proteases
topic Notes & Tips
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7094418/
https://www.ncbi.nlm.nih.gov/pubmed/21600185
http://dx.doi.org/10.1016/j.ab.2011.04.034
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