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Fast, selective and quantitative protein profiling of adenovirus-vector based vaccines by ultra-performance liquid chromatography
A method for the quantitative determination of the protein composition of adenovirus-vector based vaccines was developed. The final method used RP-UPLC with UV absorbance detection, a C4 column (300 Å, 1.7 μm, 2.1 × 150 mm), and a water- acetonitrile (ACN) gradient containing trifluoroacetic acid (T...
Autores principales: | van Tricht, Ewoud, de Raadt, Pascal, Verwilligen, Annemiek, Schenning, Martijn, Backus, Harold, Germano, Marta, Somsen, Govert W., Sänger-van de Griend, Cari E. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier B.V.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7094600/ https://www.ncbi.nlm.nih.gov/pubmed/30389208 http://dx.doi.org/10.1016/j.chroma.2018.10.045 |
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