SLAM-MS: Mutation scanning of stem-loop amplicons with TaqMan probes by quantitative DNA melting analysis

DNA Melting Analysis (DMA) with a TaqMan probe covering the mutation “hot spot” is a simple, sensitive, and “closed tube” method of mutation detection. However, DMA requires asymmetric PCR to produce single-stranded amplicons capable of interacting with TaqMan probes. This makes quantitative analysi...

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Detalles Bibliográficos
Autores principales: Kondratova, V. N., Botezatu, I. V., Shelepov, V. P., Lichtenstein, A. V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7096437/
https://www.ncbi.nlm.nih.gov/pubmed/32214156
http://dx.doi.org/10.1038/s41598-020-62173-x

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7096437/
https://www.ncbi.nlm.nih.gov/pubmed/32214156
http://dx.doi.org/10.1038/s41598-020-62173-x

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