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An Analytically and Diagnostically Sensitive RNA Extraction and RT-qPCR Protocol for Peripheral Blood Mononuclear Cells

Reliable extraction and sensitive detection of RNA from human peripheral blood mononuclear cells (PBMCs) is critical for a broad spectrum of immunology research and clinical diagnostics. RNA analysis platforms are dependent upon high-quality and high-quantity RNA; however, sensitive detection of spe...

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Detalles Bibliográficos
Autores principales: Browne, Daniel J., Brady, Jamie L., Waardenberg, Ashley J., Loiseau, Claire, Doolan, Denise L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7098950/
https://www.ncbi.nlm.nih.gov/pubmed/32265908
http://dx.doi.org/10.3389/fimmu.2020.00402