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An Analytically and Diagnostically Sensitive RNA Extraction and RT-qPCR Protocol for Peripheral Blood Mononuclear Cells
Reliable extraction and sensitive detection of RNA from human peripheral blood mononuclear cells (PBMCs) is critical for a broad spectrum of immunology research and clinical diagnostics. RNA analysis platforms are dependent upon high-quality and high-quantity RNA; however, sensitive detection of spe...
Autores principales: | Browne, Daniel J., Brady, Jamie L., Waardenberg, Ashley J., Loiseau, Claire, Doolan, Denise L. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7098950/ https://www.ncbi.nlm.nih.gov/pubmed/32265908 http://dx.doi.org/10.3389/fimmu.2020.00402 |
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