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Site-Specific Gene Knock-Out and On-Site Heterologous Gene Overexpression in Chlamydomonas reinhardtii via a CRISPR-Cas9-Mediated Knock-in Method

Chlamydomonas reinhardtii is being transformed from a model organism to an industrial organism for the production of pigments, fatty acids, and pharmaceuticals. Genetic modification has been used to increase the economic value of C. reinhardtii. However, low gene-editing efficiency and position-effe...

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Detalles Bibliográficos
Autores principales: Kim, Jongrae, Lee, Sangmuk, Baek, Kwangryul, Jin, EonSeon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7099044/
https://www.ncbi.nlm.nih.gov/pubmed/32265959
http://dx.doi.org/10.3389/fpls.2020.00306