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Engineering Insights for Multiplexed Real-Time Nucleic Acid Sequence-Based Amplification (NASBA): Implications for Design of Point-of-Care Diagnostics

BACKGROUND: Nucleic acid sequence-based amplification (NASBA) offers huge potential for low-cost, point-of-care (POC) diagnostic devices, but has been limited by high false-positive rates and the challenges of primer design. OBJECTIVE: We offer a systematic analysis of NASBA design with a view towar...

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Detalles Bibliográficos
Autores principales: Morabito, Kenneth, Wiske, Clay, Tripathi, Anubhav
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing AG 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7099912/
https://www.ncbi.nlm.nih.gov/pubmed/23677856
http://dx.doi.org/10.1007/s40291-013-0029-4