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RNAi Therapy for HIV Infection: Principles and Practicalities

Inside eukaryotic cells, small RNA duplexes, called small interfering RNAs (siRNAs), activate a conserved RNA interference (RNAi) pathway which leads to specific degradation of complementary target mRNAs through base-pairing recognition. As with other viruses, studies have shown that replication of...

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Detalles Bibliográficos
Autores principales: Bennasser, Yamina, Yeung, Man Lung, Jeang, Kuan-Teh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7100089/
https://www.ncbi.nlm.nih.gov/pubmed/17263586
http://dx.doi.org/10.2165/00063030-200721010-00003
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author Bennasser, Yamina
Yeung, Man Lung
Jeang, Kuan-Teh
author_facet Bennasser, Yamina
Yeung, Man Lung
Jeang, Kuan-Teh
author_sort Bennasser, Yamina
collection PubMed
description Inside eukaryotic cells, small RNA duplexes, called small interfering RNAs (siRNAs), activate a conserved RNA interference (RNAi) pathway which leads to specific degradation of complementary target mRNAs through base-pairing recognition. As with other viruses, studies have shown that replication of the HIV-1 in cultured cells can be targeted and inhibited by synthetic siRNAs. The relative ease of siRNA design and the versatility of RNAi to target a broad spectrum of mRNAs have led to the promise that drug discovery in the RNAi pathway could be effective against pathogens. This review discusses the current experimental principles that guide the application of RNAi against HIV and describes challenges and limitations that need to be surmounted in order for siRNAs to become practical antiviral drugs. The practical use of RNAi therapy for HIV infection will depend on overcoming several challenges, including the ability to establish long-term expression of siRNA without off-target effects and the capacity to counteract mutant escape viruses.
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spelling pubmed-71000892020-03-27 RNAi Therapy for HIV Infection: Principles and Practicalities Bennasser, Yamina Yeung, Man Lung Jeang, Kuan-Teh BioDrugs Leading Article Inside eukaryotic cells, small RNA duplexes, called small interfering RNAs (siRNAs), activate a conserved RNA interference (RNAi) pathway which leads to specific degradation of complementary target mRNAs through base-pairing recognition. As with other viruses, studies have shown that replication of the HIV-1 in cultured cells can be targeted and inhibited by synthetic siRNAs. The relative ease of siRNA design and the versatility of RNAi to target a broad spectrum of mRNAs have led to the promise that drug discovery in the RNAi pathway could be effective against pathogens. This review discusses the current experimental principles that guide the application of RNAi against HIV and describes challenges and limitations that need to be surmounted in order for siRNAs to become practical antiviral drugs. The practical use of RNAi therapy for HIV infection will depend on overcoming several challenges, including the ability to establish long-term expression of siRNA without off-target effects and the capacity to counteract mutant escape viruses. Springer International Publishing 2012-08-16 2007 /pmc/articles/PMC7100089/ /pubmed/17263586 http://dx.doi.org/10.2165/00063030-200721010-00003 Text en © Adis Data Information BV 2007 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Leading Article
Bennasser, Yamina
Yeung, Man Lung
Jeang, Kuan-Teh
RNAi Therapy for HIV Infection: Principles and Practicalities
title RNAi Therapy for HIV Infection: Principles and Practicalities
title_full RNAi Therapy for HIV Infection: Principles and Practicalities
title_fullStr RNAi Therapy for HIV Infection: Principles and Practicalities
title_full_unstemmed RNAi Therapy for HIV Infection: Principles and Practicalities
title_short RNAi Therapy for HIV Infection: Principles and Practicalities
title_sort rnai therapy for hiv infection: principles and practicalities
topic Leading Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7100089/
https://www.ncbi.nlm.nih.gov/pubmed/17263586
http://dx.doi.org/10.2165/00063030-200721010-00003
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