Knockout of secondary alcohol dehydrogenase in Nocardia cholesterolicum NRRL 5767 by CRISPR/Cas9 genome editing technology

Nocardia cholesterolicum NRRL 5767 is well-known for its ability to convert oleic acid to 10-hydroxystearic acid (~88%, w/w) and 10-ketostearic acid (~11%, w/w). Conversion of oleic acid to 10-hydroxystearic acid and then to 10-ketostearic acid has been proposed to be catalyzed by oleate hydratase a...

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Detalles Bibliográficos
Autores principales: Huang, Jenq-Kuen, Samassekou, Kadidia, Alhmadi, Hekmat B., VanDerway, David R., Diaz, Joshua D., Seiver, Jacob A., McClenahan, Shawn W., Holt, Scott M., Wen, Lisa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7101164/
https://www.ncbi.nlm.nih.gov/pubmed/32218601
http://dx.doi.org/10.1371/journal.pone.0230915

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7101164/
https://www.ncbi.nlm.nih.gov/pubmed/32218601
http://dx.doi.org/10.1371/journal.pone.0230915

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