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A set of Yarrowia lipolytica CRISPR/Cas9 vectors for exploiting wild-type strain diversity

OBJECTIVES: The construction and validation of a set of Yarrowia lipolytica CRISPR/Cas9 vectors containing six different markers that allows virtually any genetic background to be edited, including those of wild-type strains. RESULTS: Using the Golden Gate method, we assembled a set of six CRISPR/Ca...

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Detalles Bibliográficos
Autores principales: Larroude, Macarena, Trabelsi, Heykel, Nicaud, Jean-Marc, Rossignol, Tristan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7101291/
https://www.ncbi.nlm.nih.gov/pubmed/31974649
http://dx.doi.org/10.1007/s10529-020-02805-4