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A set of Yarrowia lipolytica CRISPR/Cas9 vectors for exploiting wild-type strain diversity
OBJECTIVES: The construction and validation of a set of Yarrowia lipolytica CRISPR/Cas9 vectors containing six different markers that allows virtually any genetic background to be edited, including those of wild-type strains. RESULTS: Using the Golden Gate method, we assembled a set of six CRISPR/Ca...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Springer Netherlands
2020
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7101291/ https://www.ncbi.nlm.nih.gov/pubmed/31974649 http://dx.doi.org/10.1007/s10529-020-02805-4 |