A benchmark of hemoglobin blocking during library preparation for mRNA-Sequencing of human blood samples

RNA-Sequencing (RNA-Seq) of peripheral blood can be a valuable source of information for investigating the status and mechanism of diseases. However, blood contains 50–80% unwanted hemoglobin (Hb) transcripts. Lexogen’s QuantSeq mRNA-Seq-Kit for Illumina RNA-Seq features a ‘Globin Block’ (GB) module...

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Detalles Bibliográficos
Autores principales: Uellendahl-Werth, Florian, Wolfien, Markus, Franke, Andre, Wolkenhauer, Olaf, Ellinghaus, David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7101437/
https://www.ncbi.nlm.nih.gov/pubmed/32221409
http://dx.doi.org/10.1038/s41598-020-62637-0

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7101437/
https://www.ncbi.nlm.nih.gov/pubmed/32221409
http://dx.doi.org/10.1038/s41598-020-62637-0

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