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A human microsatellite DNA-mimicking oligodeoxynucleotide with CCT repeats negatively regulates TLR7/9-mediated innate immune responses via selected TLR pathways

A human microsatellite DNA-mimicking ODN (MS ODN) composed of CCT repeats, designated as SAT05f, has been studied for its capacity of negatively regulating innate immunity induced by TLR7/TLR9 agonists in vitro and in mice. The result showed that SAT05f could down-regulate TLR7/9-dependent IFN-α pro...

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Autores principales: Sun, Ran, Sun, Luguo, Bao, Musheng, Zhang, Yongsheng, Wang, Li, Wu, Xiuli, Hu, Dali, Liu, Yongjun, Yu, Yongli, Wang, Liying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Inc. Published by Elsevier Inc. 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7106173/
https://www.ncbi.nlm.nih.gov/pubmed/20034855
http://dx.doi.org/10.1016/j.clim.2009.11.009
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author Sun, Ran
Sun, Luguo
Bao, Musheng
Zhang, Yongsheng
Wang, Li
Wu, Xiuli
Hu, Dali
Liu, Yongjun
Yu, Yongli
Wang, Liying
author_facet Sun, Ran
Sun, Luguo
Bao, Musheng
Zhang, Yongsheng
Wang, Li
Wu, Xiuli
Hu, Dali
Liu, Yongjun
Yu, Yongli
Wang, Liying
author_sort Sun, Ran
collection PubMed
description A human microsatellite DNA-mimicking ODN (MS ODN) composed of CCT repeats, designated as SAT05f, has been studied for its capacity of negatively regulating innate immunity induced by TLR7/TLR9 agonists in vitro and in mice. The result showed that SAT05f could down-regulate TLR7/9-dependent IFN-α production in cultured human PBMC stimulated by inactivated Flu virus PR8 or HSV-1 or CpG ODN or imiquimod, protect d-GalN-treated mice from lethal shock induced by TLR9 agonist, not by TLR3/4 agonist. In addition, SAT05f significantly inhibit IFN-α production from purified human plasmacytoid cells (pDCs) stimulated by CpG ODN. Interestingly, SAT05f could up-regulate CD80, CD86, and HLA-DR on the pDCs in vitro, implying that SAT05f-mediated inhibition on IFN-α production could be related to the activation of pDCs. The data suggest that SAT05f could be developed as a candidate medicament for the treatment of TLR7/9 activation-associated diseases by inhibiting TLR7/9 signaling pathways.
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spelling pubmed-71061732020-03-31 A human microsatellite DNA-mimicking oligodeoxynucleotide with CCT repeats negatively regulates TLR7/9-mediated innate immune responses via selected TLR pathways Sun, Ran Sun, Luguo Bao, Musheng Zhang, Yongsheng Wang, Li Wu, Xiuli Hu, Dali Liu, Yongjun Yu, Yongli Wang, Liying Clin Immunol Article A human microsatellite DNA-mimicking ODN (MS ODN) composed of CCT repeats, designated as SAT05f, has been studied for its capacity of negatively regulating innate immunity induced by TLR7/TLR9 agonists in vitro and in mice. The result showed that SAT05f could down-regulate TLR7/9-dependent IFN-α production in cultured human PBMC stimulated by inactivated Flu virus PR8 or HSV-1 or CpG ODN or imiquimod, protect d-GalN-treated mice from lethal shock induced by TLR9 agonist, not by TLR3/4 agonist. In addition, SAT05f significantly inhibit IFN-α production from purified human plasmacytoid cells (pDCs) stimulated by CpG ODN. Interestingly, SAT05f could up-regulate CD80, CD86, and HLA-DR on the pDCs in vitro, implying that SAT05f-mediated inhibition on IFN-α production could be related to the activation of pDCs. The data suggest that SAT05f could be developed as a candidate medicament for the treatment of TLR7/9 activation-associated diseases by inhibiting TLR7/9 signaling pathways. Elsevier Inc. Published by Elsevier Inc. 2010-03 2010-01-19 /pmc/articles/PMC7106173/ /pubmed/20034855 http://dx.doi.org/10.1016/j.clim.2009.11.009 Text en Copyright © 2009 Elsevier Inc. Published by Elsevier Inc. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Sun, Ran
Sun, Luguo
Bao, Musheng
Zhang, Yongsheng
Wang, Li
Wu, Xiuli
Hu, Dali
Liu, Yongjun
Yu, Yongli
Wang, Liying
A human microsatellite DNA-mimicking oligodeoxynucleotide with CCT repeats negatively regulates TLR7/9-mediated innate immune responses via selected TLR pathways
title A human microsatellite DNA-mimicking oligodeoxynucleotide with CCT repeats negatively regulates TLR7/9-mediated innate immune responses via selected TLR pathways
title_full A human microsatellite DNA-mimicking oligodeoxynucleotide with CCT repeats negatively regulates TLR7/9-mediated innate immune responses via selected TLR pathways
title_fullStr A human microsatellite DNA-mimicking oligodeoxynucleotide with CCT repeats negatively regulates TLR7/9-mediated innate immune responses via selected TLR pathways
title_full_unstemmed A human microsatellite DNA-mimicking oligodeoxynucleotide with CCT repeats negatively regulates TLR7/9-mediated innate immune responses via selected TLR pathways
title_short A human microsatellite DNA-mimicking oligodeoxynucleotide with CCT repeats negatively regulates TLR7/9-mediated innate immune responses via selected TLR pathways
title_sort human microsatellite dna-mimicking oligodeoxynucleotide with cct repeats negatively regulates tlr7/9-mediated innate immune responses via selected tlr pathways
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7106173/
https://www.ncbi.nlm.nih.gov/pubmed/20034855
http://dx.doi.org/10.1016/j.clim.2009.11.009
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