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Suppression of SARS-CoV entry by peptides corresponding to heptad regions on spike glycoprotein
Heptad repeat regions (HR1 and HR2) are highly conserved sequences located in the glycoproteins of enveloped viruses. They form a six-helix bundle structure and are important in the process of virus fusion. Peptides derived from the HR regions of some viruses have been shown to inhibit the entry of...
Autores principales: | , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Published by Elsevier Inc.
2004
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7111000/ https://www.ncbi.nlm.nih.gov/pubmed/15184046 http://dx.doi.org/10.1016/j.bbrc.2004.05.046 |
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author | Yuan, Kehu Yi, Ling Chen, Jian Qu, Xiuxia Qing, Tingting Rao, Xi Jiang, Pengfei Hu, Jianhe Xiong, Zikai Nie, Yuchun Shi, Xuanling Wang, Wei Ling, Chen Yin, Xiaolei Fan, Keqiang Lai, Luhua Ding, Mingxiao Deng, Hongkui |
author_facet | Yuan, Kehu Yi, Ling Chen, Jian Qu, Xiuxia Qing, Tingting Rao, Xi Jiang, Pengfei Hu, Jianhe Xiong, Zikai Nie, Yuchun Shi, Xuanling Wang, Wei Ling, Chen Yin, Xiaolei Fan, Keqiang Lai, Luhua Ding, Mingxiao Deng, Hongkui |
author_sort | Yuan, Kehu |
collection | PubMed |
description | Heptad repeat regions (HR1 and HR2) are highly conserved sequences located in the glycoproteins of enveloped viruses. They form a six-helix bundle structure and are important in the process of virus fusion. Peptides derived from the HR regions of some viruses have been shown to inhibit the entry of these viruses. SARS-CoV was also predicted to have HR1 and HR2 regions in the S2 protein. Based on this prediction, we designed 25 peptides and screened them using a HIV-luc/SARS pseudotyped virus assay. Two peptides, HR1-1 and HR2-18, were identified as potential inhibitors, with EC(50) values of 0.14 and 1.19 μM, respectively. The inhibitory effects of these peptides were validated by the wild-type SARS-CoV assay. HR1-1 and HR2-18 can serve as functional probes for dissecting the fusion mechanism of SARS-CoV and also provide the potential of further identifying potent inhibitors for SARS-CoV entry. |
format | Online Article Text |
id | pubmed-7111000 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2004 |
publisher | Published by Elsevier Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71110002020-04-02 Suppression of SARS-CoV entry by peptides corresponding to heptad regions on spike glycoprotein Yuan, Kehu Yi, Ling Chen, Jian Qu, Xiuxia Qing, Tingting Rao, Xi Jiang, Pengfei Hu, Jianhe Xiong, Zikai Nie, Yuchun Shi, Xuanling Wang, Wei Ling, Chen Yin, Xiaolei Fan, Keqiang Lai, Luhua Ding, Mingxiao Deng, Hongkui Biochem Biophys Res Commun Article Heptad repeat regions (HR1 and HR2) are highly conserved sequences located in the glycoproteins of enveloped viruses. They form a six-helix bundle structure and are important in the process of virus fusion. Peptides derived from the HR regions of some viruses have been shown to inhibit the entry of these viruses. SARS-CoV was also predicted to have HR1 and HR2 regions in the S2 protein. Based on this prediction, we designed 25 peptides and screened them using a HIV-luc/SARS pseudotyped virus assay. Two peptides, HR1-1 and HR2-18, were identified as potential inhibitors, with EC(50) values of 0.14 and 1.19 μM, respectively. The inhibitory effects of these peptides were validated by the wild-type SARS-CoV assay. HR1-1 and HR2-18 can serve as functional probes for dissecting the fusion mechanism of SARS-CoV and also provide the potential of further identifying potent inhibitors for SARS-CoV entry. Published by Elsevier Inc. 2004-07-02 2004-05-21 /pmc/articles/PMC7111000/ /pubmed/15184046 http://dx.doi.org/10.1016/j.bbrc.2004.05.046 Text en Copyright © 2004 Published by Elsevier Inc. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Yuan, Kehu Yi, Ling Chen, Jian Qu, Xiuxia Qing, Tingting Rao, Xi Jiang, Pengfei Hu, Jianhe Xiong, Zikai Nie, Yuchun Shi, Xuanling Wang, Wei Ling, Chen Yin, Xiaolei Fan, Keqiang Lai, Luhua Ding, Mingxiao Deng, Hongkui Suppression of SARS-CoV entry by peptides corresponding to heptad regions on spike glycoprotein |
title | Suppression of SARS-CoV entry by peptides corresponding to heptad regions on spike glycoprotein |
title_full | Suppression of SARS-CoV entry by peptides corresponding to heptad regions on spike glycoprotein |
title_fullStr | Suppression of SARS-CoV entry by peptides corresponding to heptad regions on spike glycoprotein |
title_full_unstemmed | Suppression of SARS-CoV entry by peptides corresponding to heptad regions on spike glycoprotein |
title_short | Suppression of SARS-CoV entry by peptides corresponding to heptad regions on spike glycoprotein |
title_sort | suppression of sars-cov entry by peptides corresponding to heptad regions on spike glycoprotein |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7111000/ https://www.ncbi.nlm.nih.gov/pubmed/15184046 http://dx.doi.org/10.1016/j.bbrc.2004.05.046 |
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