Development of a duplex real-time RT-qPCR assay to monitor genome replication, gene expression and gene insert stability during in vivo replication of a prototype live attenuated canine distemper virus vector encoding SIV gag

Advancement of new vaccines based on live viral vectors requires sensitive assays to analyze in vivo replication, gene expression and genetic stability. In this study, attenuated canine distemper virus (CDV) was used as a vaccine delivery vector and duplex 2-step quantitative real-time RT-PCR (RT-qP...

Descripción completa

Detalles Bibliográficos
Autores principales: Coleman, John W., Wright, Kevin J., Wallace, Olivia L., Sharma, Palka, Arendt, Heather, Martinez, Jennifer, DeStefano, Joanne, Zamb, Timothy P., Zhang, Xinsheng, Parks, Christopher L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7111484/
https://www.ncbi.nlm.nih.gov/pubmed/25486083
http://dx.doi.org/10.1016/j.jviromet.2014.11.015

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7111484/
https://www.ncbi.nlm.nih.gov/pubmed/25486083
http://dx.doi.org/10.1016/j.jviromet.2014.11.015

Ejemplares similares