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Use of Sequence-Independent, Single-Primer-Amplification (SISPA) for rapid detection, identification, and characterization of avian RNA viruses

Current technologies with next generation sequencing have revolutionized metagenomics analysis of clinical samples. To achieve the non-selective amplification and recovery of low abundance genetic sequences, a simplified Sequence-Independent, Single-Primer Amplification (SISPA) technique in combinat...

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Detalles Bibliográficos
Autores principales: Chrzastek, Klaudia, Lee, Dong-hun, Smith, Diane, Sharma, Poonam, Suarez, David L., Pantin-Jackwood, Mary, Kapczynski, Darrell R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Academic Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7111618/
https://www.ncbi.nlm.nih.gov/pubmed/28646651
http://dx.doi.org/10.1016/j.virol.2017.06.019