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Rapid differentiation of avian infectious bronchitis virus isolates by sample to residual ratio quantitation using real-time reverse transcriptase-polymerase chain reaction

A rapid diagnostic assay for differentiating avian infectious bronchitis virus (IBV) isolates was developed. The basis of the assay is the cleavage of target RNA by RNase H mediated by sequence-specific chimeric oligonucleotides followed by sample to residual ratio quantitation (SRRQ) using RRT-PCR....

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Detalles Bibliográficos
Autores principales: Callison, S.A., Hilt, D.A., Jackwood, M.W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7112770/
https://www.ncbi.nlm.nih.gov/pubmed/15664067
http://dx.doi.org/10.1016/j.jviromet.2004.11.022