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Detection and differentiation of virulent and avirulent strains of Newcastle disease virus by real-time PCR
A rapid diagnostic method based on the melting curve SYBR Green I real-time PCR analysis was developed to detect and differentiate Newcastle disease virus (NDV) strains. Degenerated primers based on the cleavage site sequence of the F0 gene were designed to detect specific sequences characteristic o...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier B.V.
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7112782/ https://www.ncbi.nlm.nih.gov/pubmed/21192979 http://dx.doi.org/10.1016/j.jviromet.2010.12.015 |