Development of a real-time TaqMan(®) RT-PCR assay for the detection of infectious bronchitis virus in chickens, and comparison of RT-PCR and virus isolation

A sensitive and specific method for the diagnosis of infectious bronchitis virus (IBV) is of great importance. In this study the development of a real-time TaqMan(®) RT-PCR targeting the highly conserved nucleocapsid (N) gene of IBV and including an internal PCR control is described. The assay was s...

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Detalles Bibliográficos
Autores principales: Meir, Rosie, Maharat, Ora, Farnushi, Ygal, Simanov, Lubov
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2010
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7112792/
https://www.ncbi.nlm.nih.gov/pubmed/19781572
http://dx.doi.org/10.1016/j.jviromet.2009.09.014

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7112792/
https://www.ncbi.nlm.nih.gov/pubmed/19781572
http://dx.doi.org/10.1016/j.jviromet.2009.09.014

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