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Development of a real-time TaqMan(®) RT-PCR assay for the detection of infectious bronchitis virus in chickens, and comparison of RT-PCR and virus isolation
A sensitive and specific method for the diagnosis of infectious bronchitis virus (IBV) is of great importance. In this study the development of a real-time TaqMan(®) RT-PCR targeting the highly conserved nucleocapsid (N) gene of IBV and including an internal PCR control is described. The assay was s...
Autores principales: | Meir, Rosie, Maharat, Ora, Farnushi, Ygal, Simanov, Lubov |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier B.V.
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7112792/ https://www.ncbi.nlm.nih.gov/pubmed/19781572 http://dx.doi.org/10.1016/j.jviromet.2009.09.014 |
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