Development of a one step real time RT-PCR assay to detect and quantify Dugbe virus

A one-step real time quantitative RT-PCR (qRT-PCR) assay was developed to detect all published Dugbe virus (DUGV) genomes of the Nairovirus genus. Primers and probes were designed to detect specific sequences on the most conserved regions of the S segment. The limit of detection of the assay was 10...

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Detalles Bibliográficos
Autores principales: Rodrigues, R., Telles, J.-N., Essere, K., Ducournau, C., Roqueplo, C., Levieuge, A., Davoust, B., Parola, P., Paranhos-Baccalà, G., Peyrefitte, C.N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7112838/
https://www.ncbi.nlm.nih.gov/pubmed/21703306
http://dx.doi.org/10.1016/j.jviromet.2011.06.003

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7112838/
https://www.ncbi.nlm.nih.gov/pubmed/21703306
http://dx.doi.org/10.1016/j.jviromet.2011.06.003

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