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A real-time TaqMan(®) RT-PCR assay with an internal amplification control for rapid detection of transmissible gastroenteritis virus in swine fecal samples

A TaqMan probe-based real-time RT-PCR assay was developed for simultaneous detection of RNA of transmissible gastroenteritis virus (TGEV) in pig fecal samples and RNA of enhanced green fluorescent protein (EGFP) added exogenously as an internal amplification control. The TGEV primers and probe were...

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Detalles Bibliográficos
Autores principales: Vemulapalli, Ramesh, Gulani, Jatinder, Santrich, Cecilia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7112873/
https://www.ncbi.nlm.nih.gov/pubmed/19729039
http://dx.doi.org/10.1016/j.jviromet.2009.08.016