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A real-time TaqMan(®) RT-PCR assay with an internal amplification control for rapid detection of transmissible gastroenteritis virus in swine fecal samples
A TaqMan probe-based real-time RT-PCR assay was developed for simultaneous detection of RNA of transmissible gastroenteritis virus (TGEV) in pig fecal samples and RNA of enhanced green fluorescent protein (EGFP) added exogenously as an internal amplification control. The TGEV primers and probe were...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier B.V.
2009
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7112873/ https://www.ncbi.nlm.nih.gov/pubmed/19729039 http://dx.doi.org/10.1016/j.jviromet.2009.08.016 |