Cargando…
The attenuation of vaccinia Tian Tan strain by the removal of the viral M1L-K2L genes
To generate a safe vaccinia Tian Tan (VTT)-based vaccine vector, it is necessary to develop a method to attenuate the virus. A modified VTT (MVTT(2-GFP)) was constructed by replacing the viral M1L-K2L genes with a GFP gene. In comparison to the parental VTT, MVTT(2-GFP) lost its replication capacity...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier B.V.
2007
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7112875/ https://www.ncbi.nlm.nih.gov/pubmed/17459491 http://dx.doi.org/10.1016/j.jviromet.2007.03.012 |
_version_ | 1783513562521534464 |
---|---|
author | Zhu, Weijun Fang, Qing Zhuang, Ke Wang, Haibo Yu, Wenbo Zhou, Jingying Liu, Li Tien, Po Zhang, Linqi Chen, Zhiwei |
author_facet | Zhu, Weijun Fang, Qing Zhuang, Ke Wang, Haibo Yu, Wenbo Zhou, Jingying Liu, Li Tien, Po Zhang, Linqi Chen, Zhiwei |
author_sort | Zhu, Weijun |
collection | PubMed |
description | To generate a safe vaccinia Tian Tan (VTT)-based vaccine vector, it is necessary to develop a method to attenuate the virus. A modified VTT (MVTT(2-GFP)) was constructed by replacing the viral M1L-K2L genes with a GFP gene. In comparison to the parental VTT, MVTT(2-GFP) lost its replication capacity in rabbit RK13 and human HeLa cell lines. The life cycle of viral replication was blocked at different stages in these two cell lines as determined by electron microscope examination. MVTT(2-GFP) was less virulent than VTT for 100-fold by measuring mouse body weight loss after intranasal viral inoculation and for 340-fold by determining the intracranial LD(50) value in mice. The foreign GFP gene was stable genetically after 10 rounds of passage in Vero cells. Importantly, MVTT(2-GFP) elicited both humoral and cell-mediated immune responses to the GFP gene in mice. With two intramuscular inoculations of 10(5) PFU virus, the anti-GFP antibody reciprocal endpoint titer reached over 700 as determined by an ELISA. The number of IFN-γ secreting T cells reached over 350 SFU per million splenocytes against a CD8+ T cell-specific epitope of GFP. Collectively, the removal of the M1L-K2L genes is a useful method to generate an attenuated vaccinia Tian Tan vaccine vector. |
format | Online Article Text |
id | pubmed-7112875 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
publisher | Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71128752020-04-02 The attenuation of vaccinia Tian Tan strain by the removal of the viral M1L-K2L genes Zhu, Weijun Fang, Qing Zhuang, Ke Wang, Haibo Yu, Wenbo Zhou, Jingying Liu, Li Tien, Po Zhang, Linqi Chen, Zhiwei J Virol Methods Article To generate a safe vaccinia Tian Tan (VTT)-based vaccine vector, it is necessary to develop a method to attenuate the virus. A modified VTT (MVTT(2-GFP)) was constructed by replacing the viral M1L-K2L genes with a GFP gene. In comparison to the parental VTT, MVTT(2-GFP) lost its replication capacity in rabbit RK13 and human HeLa cell lines. The life cycle of viral replication was blocked at different stages in these two cell lines as determined by electron microscope examination. MVTT(2-GFP) was less virulent than VTT for 100-fold by measuring mouse body weight loss after intranasal viral inoculation and for 340-fold by determining the intracranial LD(50) value in mice. The foreign GFP gene was stable genetically after 10 rounds of passage in Vero cells. Importantly, MVTT(2-GFP) elicited both humoral and cell-mediated immune responses to the GFP gene in mice. With two intramuscular inoculations of 10(5) PFU virus, the anti-GFP antibody reciprocal endpoint titer reached over 700 as determined by an ELISA. The number of IFN-γ secreting T cells reached over 350 SFU per million splenocytes against a CD8+ T cell-specific epitope of GFP. Collectively, the removal of the M1L-K2L genes is a useful method to generate an attenuated vaccinia Tian Tan vaccine vector. Elsevier B.V. 2007-09 2007-04-24 /pmc/articles/PMC7112875/ /pubmed/17459491 http://dx.doi.org/10.1016/j.jviromet.2007.03.012 Text en Copyright © 2007 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Zhu, Weijun Fang, Qing Zhuang, Ke Wang, Haibo Yu, Wenbo Zhou, Jingying Liu, Li Tien, Po Zhang, Linqi Chen, Zhiwei The attenuation of vaccinia Tian Tan strain by the removal of the viral M1L-K2L genes |
title | The attenuation of vaccinia Tian Tan strain by the removal of the viral M1L-K2L genes |
title_full | The attenuation of vaccinia Tian Tan strain by the removal of the viral M1L-K2L genes |
title_fullStr | The attenuation of vaccinia Tian Tan strain by the removal of the viral M1L-K2L genes |
title_full_unstemmed | The attenuation of vaccinia Tian Tan strain by the removal of the viral M1L-K2L genes |
title_short | The attenuation of vaccinia Tian Tan strain by the removal of the viral M1L-K2L genes |
title_sort | attenuation of vaccinia tian tan strain by the removal of the viral m1l-k2l genes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7112875/ https://www.ncbi.nlm.nih.gov/pubmed/17459491 http://dx.doi.org/10.1016/j.jviromet.2007.03.012 |
work_keys_str_mv | AT zhuweijun theattenuationofvacciniatiantanstrainbytheremovaloftheviralm1lk2lgenes AT fangqing theattenuationofvacciniatiantanstrainbytheremovaloftheviralm1lk2lgenes AT zhuangke theattenuationofvacciniatiantanstrainbytheremovaloftheviralm1lk2lgenes AT wanghaibo theattenuationofvacciniatiantanstrainbytheremovaloftheviralm1lk2lgenes AT yuwenbo theattenuationofvacciniatiantanstrainbytheremovaloftheviralm1lk2lgenes AT zhoujingying theattenuationofvacciniatiantanstrainbytheremovaloftheviralm1lk2lgenes AT liuli theattenuationofvacciniatiantanstrainbytheremovaloftheviralm1lk2lgenes AT tienpo theattenuationofvacciniatiantanstrainbytheremovaloftheviralm1lk2lgenes AT zhanglinqi theattenuationofvacciniatiantanstrainbytheremovaloftheviralm1lk2lgenes AT chenzhiwei theattenuationofvacciniatiantanstrainbytheremovaloftheviralm1lk2lgenes AT zhuweijun attenuationofvacciniatiantanstrainbytheremovaloftheviralm1lk2lgenes AT fangqing attenuationofvacciniatiantanstrainbytheremovaloftheviralm1lk2lgenes AT zhuangke attenuationofvacciniatiantanstrainbytheremovaloftheviralm1lk2lgenes AT wanghaibo attenuationofvacciniatiantanstrainbytheremovaloftheviralm1lk2lgenes AT yuwenbo attenuationofvacciniatiantanstrainbytheremovaloftheviralm1lk2lgenes AT zhoujingying attenuationofvacciniatiantanstrainbytheremovaloftheviralm1lk2lgenes AT liuli attenuationofvacciniatiantanstrainbytheremovaloftheviralm1lk2lgenes AT tienpo attenuationofvacciniatiantanstrainbytheremovaloftheviralm1lk2lgenes AT zhanglinqi attenuationofvacciniatiantanstrainbytheremovaloftheviralm1lk2lgenes AT chenzhiwei attenuationofvacciniatiantanstrainbytheremovaloftheviralm1lk2lgenes |