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Real-time RT-PCR detection of Bovine Viral Diarrhoea virus in whole blood using an external RNA reference
A novel two-step real-time RT-PCR assay using SYBR(®) Green I was developed for the detection of acute Bovine Viral Diarrhoea virus (BVDV) infection in whole blood from cattle. During infection animals experience a characteristic transient leucopenia and the number of cells per volume of blood chang...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier B.V.
2006
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7112878/ https://www.ncbi.nlm.nih.gov/pubmed/17030066 http://dx.doi.org/10.1016/j.jviromet.2006.08.008 |