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Real-time RT-PCR detection of Bovine Viral Diarrhoea virus in whole blood using an external RNA reference

A novel two-step real-time RT-PCR assay using SYBR(®) Green I was developed for the detection of acute Bovine Viral Diarrhoea virus (BVDV) infection in whole blood from cattle. During infection animals experience a characteristic transient leucopenia and the number of cells per volume of blood chang...

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Detalles Bibliográficos
Autores principales: Young, N.J., Thomas, C.J., Collins, M.E., Brownlie, J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7112878/
https://www.ncbi.nlm.nih.gov/pubmed/17030066
http://dx.doi.org/10.1016/j.jviromet.2006.08.008