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Development of a reverse transcription multiplex real-time PCR for the detection and genotyping of classical swine fever virus

A reverse transcription multiplex real-time PCR (RT-MRT-PCR) was developed for rapid detection and genotyping of classical swine fever virus (CSFV). The universal primers and specific TaqMan probes for each of the three genotypes, genotypes 1, 2, and 3, were designed within the 3′-UTR of the CSFV. N...

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Detalles Bibliográficos
Autores principales: Huang, Yu-Liang, Pang, Victor Fei, Pan, Chu-Hsiang, Chen, Tsu-Han, Jong, Ming-Hwa, Huang, Tien-Shine, Jeng, Chian-Ren
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7112934/
https://www.ncbi.nlm.nih.gov/pubmed/19414034
http://dx.doi.org/10.1016/j.jviromet.2009.04.029