Effective CRISPRa-mediated control of gene expression in bacteria must overcome strict target site requirements

In bacterial systems, CRISPR-Cas transcriptional activation (CRISPRa) has the potential to dramatically expand our ability to regulate gene expression, but we lack predictive rules for designing effective gRNA target sites. Here, we identify multiple features of bacterial promoters that impose strin...

Descripción completa

Detalles Bibliográficos
Autores principales: Fontana, Jason, Dong, Chen, Kiattisewee, Cholpisit, Chavali, Venkata P., Tickman, Benjamin I., Carothers, James M., Zalatan, Jesse G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7113249/
https://www.ncbi.nlm.nih.gov/pubmed/32238808
http://dx.doi.org/10.1038/s41467-020-15454-y

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7113249/
https://www.ncbi.nlm.nih.gov/pubmed/32238808
http://dx.doi.org/10.1038/s41467-020-15454-y

Ejemplares similares