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Development of fluorescent reverse transcription loop-mediated isothermal amplification (RT-LAMP) using quenching probes for the detection of the Middle East respiratory syndrome coronavirus

Clinical detection of Middle East respiratory syndrome (MERS) coronavirus (MERS-CoV) in patients is achieved using genetic diagnostic methods, such as real-time RT-PCR assay. Previously, we developed a reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay for the detection of...

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Detalles Bibliográficos
Autores principales: Shirato, Kazuya, Semba, Shohei, El-Kafrawy, Sherif A., Hassan, Ahmed M., Tolah, Ahmed M., Takayama, Ikuyo, Kageyama, Tsutomu, Notomi, Tsugunori, Kamitani, Wataru, Matsuyama, Shutoku, Azhar, Esam Ibraheem
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Authors. Published by Elsevier B.V. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7113683/
https://www.ncbi.nlm.nih.gov/pubmed/29763640
http://dx.doi.org/10.1016/j.jviromet.2018.05.006