Expression, purification, and in vitro activity of an arterivirus main proteinase

To allow the biochemical and structural characterization of the chymotrypsin-like “main proteinase” (non-structural protein 4; nsp4) of the arterivirus prototype Equine Arteritis Virus (EAV), we developed protocols for the large-scale production of recombinant nsp4 in Escherichia coli. The nsp4 prot...

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Detalles Bibliográficos
Autores principales: van Aken, Danny, Benckhuijsen, Willemien E., Drijfhout, Jan W., Wassenaar, Alfred L.M., Gorbalenya, Alexander E., Snijder, Eric J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2006
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7114227/
https://www.ncbi.nlm.nih.gov/pubmed/16527369
http://dx.doi.org/10.1016/j.virusres.2006.01.025

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7114227/
https://www.ncbi.nlm.nih.gov/pubmed/16527369
http://dx.doi.org/10.1016/j.virusres.2006.01.025

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