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Expression, purification, and in vitro activity of an arterivirus main proteinase
To allow the biochemical and structural characterization of the chymotrypsin-like “main proteinase” (non-structural protein 4; nsp4) of the arterivirus prototype Equine Arteritis Virus (EAV), we developed protocols for the large-scale production of recombinant nsp4 in Escherichia coli. The nsp4 prot...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier B.V.
2006
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7114227/ https://www.ncbi.nlm.nih.gov/pubmed/16527369 http://dx.doi.org/10.1016/j.virusres.2006.01.025 |