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Expression and functional analysis of porcine aminopeptidase N produced in prokaryotic expression system

Porcine aminopeptidase N (pAPN) is a cellular membrane protein and a functional receptor for porcine coronaviruses. Here, we describe the heterologous expression of pAPN without signal peptide in BL21(DE3)pLysS host cells. The Escherichia coli (E. coli) harboring the recombinant construct was effici...

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Detalles Bibliográficos
Autores principales: Liu, Boqi, Li, Guangxing, Sui, Xiuwen, Yin, Jiechao, Wang, Heng, Ren, Xiaofeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7114256/
https://www.ncbi.nlm.nih.gov/pubmed/19428736
http://dx.doi.org/10.1016/j.jbiotec.2009.02.005