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Expression and functional analysis of porcine aminopeptidase N produced in prokaryotic expression system
Porcine aminopeptidase N (pAPN) is a cellular membrane protein and a functional receptor for porcine coronaviruses. Here, we describe the heterologous expression of pAPN without signal peptide in BL21(DE3)pLysS host cells. The Escherichia coli (E. coli) harboring the recombinant construct was effici...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier B.V.
2009
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7114256/ https://www.ncbi.nlm.nih.gov/pubmed/19428736 http://dx.doi.org/10.1016/j.jbiotec.2009.02.005 |