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Fluorescence Photobleaching as an Intrinsic Tool to Quantify the 3D Expansion Factor of Biological Samples in Expansion Microscopy

[Image: see text] Four years after its first report, expansion microscopy (ExM) is now being routinely applied in laboratories worldwide to achieve super-resolution imaging on conventional fluorescence microscopes. By chemically anchoring all molecules of interest to the polymer meshwork of an expan...

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Detalles Bibliográficos
Autores principales: Vanheusden, Marisa, Vitale, Raffaele, Camacho, Rafael, Janssen, Kris P. F., Acke, Aline, Rocha, Susana, Hofkens, Johan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2020
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7114699/
https://www.ncbi.nlm.nih.gov/pubmed/32258914
http://dx.doi.org/10.1021/acsomega.0c00118