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Single Cell Glucose Uptake Assays: A Cautionary Tale

Assays to monitor the metabolic state or nutrient uptake capacity of immune cells at a single cell level are increasingly in demand. One assay, used by many immunologists, employs 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG), a fluorescent analogue of 2-deoxyglucose (2DG),...

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Detalles Bibliográficos
Autores principales: Sinclair, Linda V., Barthelemy, Celine, Cantrell, Doreen A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7116014/
https://www.ncbi.nlm.nih.gov/pubmed/32879737
http://dx.doi.org/10.20900/immunometab20200029