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Development of protein A-gold immunoelectron microscopy for detection of bovine coronavirus in calves: Comparison with ELISA and direct immunofluorescence of nasal epithelial cells()

A protein A-colloidal gold immunoelectron microscopy (PAG-IEM) technique was developed for the detection of bovine coronavirus (BCV) in the feces and nasal secretions of infected calves. Feces or nasal swab fluids were incubated sequentially with hyperimmune bovine anti-bovine coronavirus serum and...

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Detalles Bibliográficos
Autores principales: Heckert, R.A., Saif, L.J., Myers, G.W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier B.V. 1989
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7117413/
https://www.ncbi.nlm.nih.gov/pubmed/2541532
http://dx.doi.org/10.1016/0378-1135(89)90068-0