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Development of protein A-gold immunoelectron microscopy for detection of bovine coronavirus in calves: Comparison with ELISA and direct immunofluorescence of nasal epithelial cells()
A protein A-colloidal gold immunoelectron microscopy (PAG-IEM) technique was developed for the detection of bovine coronavirus (BCV) in the feces and nasal secretions of infected calves. Feces or nasal swab fluids were incubated sequentially with hyperimmune bovine anti-bovine coronavirus serum and...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Published by Elsevier B.V.
1989
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7117413/ https://www.ncbi.nlm.nih.gov/pubmed/2541532 http://dx.doi.org/10.1016/0378-1135(89)90068-0 |