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Enzyme-linked immunosorbent assay for the detection of porcine epidemic diarrhea coronavirus antibodies in swine sera
An enzyme-linked immunosorbent assay (ELISA) for detecting serum antibodies to the porcine epidemic diarrhea coronavirus (PEDV) was established by using cell culture-grown PEDV as antigen for coating. Ultracentrifugation through 20 and 45% (w/w) sucrose cushion proved to be the best antigen purifica...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Published by Elsevier B.V.
1990
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7117443/ https://www.ncbi.nlm.nih.gov/pubmed/2154877 http://dx.doi.org/10.1016/0378-1135(90)90037-V |