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Enzyme-linked immunosorbent assay for the detection of porcine epidemic diarrhea coronavirus antibodies in swine sera

An enzyme-linked immunosorbent assay (ELISA) for detecting serum antibodies to the porcine epidemic diarrhea coronavirus (PEDV) was established by using cell culture-grown PEDV as antigen for coating. Ultracentrifugation through 20 and 45% (w/w) sucrose cushion proved to be the best antigen purifica...

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Detalles Bibliográficos
Autores principales: Hofmann, Martin, Wyler, Robert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier B.V. 1990
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7117443/
https://www.ncbi.nlm.nih.gov/pubmed/2154877
http://dx.doi.org/10.1016/0378-1135(90)90037-V