Development and optimization of plaque assays for rat coronaviruses

Plaque assays under Sephadex or agarose overlays are described for rat coronaviruses (RCVs) grown in L2 mouse fibroblasts. A plaque assay using Sephadex was simple; however, viable plaques could not be collected for propagation, and fixation was necessary before evaluation. Plaque formation under ag...

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Detalles Bibliográficos
Autores principales: Gaertner, D.J., Winograd, D.F., Compton, S.R., Paturzo, F.X., Smith, A.L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier B.V. 1993
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7119490/
https://www.ncbi.nlm.nih.gov/pubmed/7689580
http://dx.doi.org/10.1016/0166-0934(93)90089-A

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7119490/
https://www.ncbi.nlm.nih.gov/pubmed/7689580
http://dx.doi.org/10.1016/0166-0934(93)90089-A

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