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Development and optimization of plaque assays for rat coronaviruses
Plaque assays under Sephadex or agarose overlays are described for rat coronaviruses (RCVs) grown in L2 mouse fibroblasts. A plaque assay using Sephadex was simple; however, viable plaques could not be collected for propagation, and fixation was necessary before evaluation. Plaque formation under ag...
Autores principales: | Gaertner, D.J., Winograd, D.F., Compton, S.R., Paturzo, F.X., Smith, A.L. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Published by Elsevier B.V.
1993
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7119490/ https://www.ncbi.nlm.nih.gov/pubmed/7689580 http://dx.doi.org/10.1016/0166-0934(93)90089-A |
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