Cargando…
Optimization of extraction and PCR amplification of RNA extracts from paraffin-embedded tissue in different fixatives
A method was developed for fast and efficient isolation of RNA from paraffin-embedded tissue sections for subsequent PCR analysis. This method is based on the binding of RNA to acid-treated glass beads in the presence of a high molarity of guanidinium salt. It can be completed within an hour, and ob...
| Autores principales: | , , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Published by Elsevier B.V.
1993
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7119522/ https://www.ncbi.nlm.nih.gov/pubmed/8396155 http://dx.doi.org/10.1016/0166-0934(93)90076-4 |