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Quantitation of respiratory syncytial virus RNA in nasal aspirates of children by real-time RT-PCR assay
A method was developed for the quantitation of respiratory syncytial virus (RSV) based on real-time RT-PCR using a LightCycler instrument. A control real-time RT-PCR was undertaken on GAPDH mRNA (a human housekeeping gene) was carried out to standardise the non-homogeneous respiratory samples. The r...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier Science B.V.
2003
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7119593/ https://www.ncbi.nlm.nih.gov/pubmed/12668266 http://dx.doi.org/10.1016/S0166-0934(03)00042-9 |