Cargando…

Quantitation of respiratory syncytial virus RNA in nasal aspirates of children by real-time RT-PCR assay

A method was developed for the quantitation of respiratory syncytial virus (RSV) based on real-time RT-PCR using a LightCycler instrument. A control real-time RT-PCR was undertaken on GAPDH mRNA (a human housekeeping gene) was carried out to standardise the non-homogeneous respiratory samples. The r...

Descripción completa

Detalles Bibliográficos
Autores principales:	Gueudin, M, Vabret, A, Petitjean, J, Gouarin, S, Brouard, J, Freymuth, F
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier Science B.V. 2003
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7119593/ https://www.ncbi.nlm.nih.gov/pubmed/12668266 http://dx.doi.org/10.1016/S0166-0934(03)00042-9

Ejemplares similares

Development of three multiplex RT-PCR assays for the detection of 12 respiratory RNA viruses
por: Bellau-Pujol, S., et al.
Publicado: (2005)

Development of duplex real-time PCR for detection of two DNA respiratory viruses
por: Dina, Julia, et al.
Publicado: (2009)

Development of a PCR-and hybridization-based assay (PCR Adenovirus Consensus(®)) for the detection and the species identification of adenoviruses in respiratory specimens
por: Vabret, Astrid, et al.
Publicado: (2004)

Le virus respiratoire syncytial: importance en pathologie, méthodes diagnostiques, traitement et prévention
por: Freymuth, François, et al.
Publicado: (1995)

Infections à Mycoplasma pneumoniae : étude rétrospective en Basse-Normandie de 1997 à 2005. Épidémiologie –– Place de la sérologie et de la PCR pour le diagnostic
por: Petitjean-Lecherbonnier, J., et al.
Publicado: (2006)

La grippe saisonnière
por: Vabret, A., et al.
Publicado: (2010)

Exacerbations de l’asthme et identification virale associée à celle du Chlamydiae pneumoniae et du Mycoplasma pneumoniae : épidémiologie comparative entre nourrissons et enfants âgés de plus de deux ans
por: Brouard, J, et al.
Publicado: (2001)

Épidémiologie de l'infection virale et asthme
por: Freymuth, F., et al.
Publicado: (1998)

Direct diagnosis of human respiratory coronaviruses 229E and OC43 by the polymerase chain reaction
por: Vabret, Astrid, et al.
Publicado: (2001)

Human Coronavirus NL63, France
por: Vabret, Astrid, et al.
Publicado: (2005)

Detection of viral, Chlamydia pneumoniae and Mycoplasma pneumoniae infections in exacerbations of asthma in children
por: Freymuth, François, et al.
Publicado: (1999)

Techniques actuelles de diagnostic des infections virales respiratoires en réanimation
por: Freymuth, F., et al.
Publicado: (2007)

Épidémiologie et diagnostic des infections à virus respiratoire syncytial de l’adulte
por: Freymuth, F., et al.
Publicado: (2004)

Métapneumovirus humain
por: Freymuth, F., et al.
Publicado: (2009)

Asthme du nourrisson : monopole du virus respiratoire syncytial ?
por: Brouard, J., et al.
Publicado: (2006)

Les virus des bronchiolites aiguës
por: Freymuth, F., et al.
Publicado: (2010)

Evaluation of quantitative and type-specific real-time RT-PCR assays for detection of respiratory syncytial virus in respiratory specimens from children
por: Kuypers, Jane, et al.
Publicado: (2004)

Duplex real-time RT-PCR assay for detection and subgroup-specific identification of human respiratory syncytial virus
por: Wang, Lijuan, et al.
Publicado: (2019)

Comparison of Direct Fluorescence Assay and Real-Time RT-PCR as Diagnostics for Respiratory Syncytial Virus in Young Children
por: Shafik, Caroline F., et al.
Publicado: (2011)

Real-Time RT-PCR Detection of Equine Coronavirus
por: Miszczak, Fabien, et al.
Publicado: (2015)

Performance Evaluation of the VIDAS(®) Measles IgG Assay and Its Diagnostic Value for Measuring IgG Antibody Avidity in Measles Virus Infection
por: Dina, Julia, et al.
Publicado: (2016)

Diagnostic moléculaire des infections respiratoires à entérovirus : apport de la PCR et du génotypage pour une meilleure approche de la circulation des souches en Basse-Normandie au cours de l’année 2008
por: Petitjean-Lecherbonnier, J., et al.
Publicado: (2011)

Bien évaluer l'épidémie à VRS
por: Brouard, Jacques, et al.
Publicado: (2006)

A quantitative real-time RT-PCR assay for mature C. albicans biofilms
por: Xie, Zhihong, et al.
Publicado: (2011)

Rapid and sensitive real-time assay for the detection of respiratory syncytial virus using RT-SIBA®
por: Eboigbodin, Kevin E., et al.
Publicado: (2017)

Two RT-PCR based assays to detect human metapneumovirus in nasopharyngeal aspirates
por: López-Huertas, María Rosa, et al.
Publicado: (2005)

Evaluation of Analytical and Clinical Performance and Usefulness in a Real-Life Hospital Setting of Two in-House Real-Time RT-PCR Assays to Track SARS-CoV-2 Variants of Concern
por: Moisan, Alice, et al.
Publicado: (2023)

Detection of respiratory syncytial virus infection in nasal aspirate samples by flow cytometry
por: Johnston, S.L., et al.
Publicado: (1994)

Coronavirus humains (HCoV)
por: Vabret, A., et al.
Publicado: (2009)

A multiplex one-tube nested real time RT-PCR assay for simultaneous detection of respiratory syncytial virus, human rhinovirus and human metapneumovirus
por: Feng, Zhi-shan, et al.
Publicado: (2018)

A highly sensitive 1-tube nested real-time RT-PCR assay using LNA-modified primers for detection of respiratory syncytial virus
por: Zhao, Li, et al.
Publicado: (2019)

Evaluation of a single-tube fluorogenic RT-PCR assay for detection of bovine respiratory syncytial virus in clinical samples
por: Hakhverdyan, Mikhayil, et al.
Publicado: (2005)

A Real-Time RT-PCR Assay for Genotyping of Rotavirus
por: Mousavi-Nasab, Seyed Dawood, et al.
Publicado: (2020)

Typing and subtyping of influenza viruses and respiratory syncytial viruses by multiplex RT-PCR
por: Yamada, Akira, et al.
Publicado: (2004)

Whole-genome analysis of pseudorabies virus gene expression by real-time quantitative RT-PCR assay
por: Tombácz, Dóra, et al.
Publicado: (2009)

Development of a Real-Time Quantitative RT-PCR Assay for Detection of Bovine Rhinitis B Virus
por: Xie, Yi-Lun, et al.
Publicado: (2021)

Allergie respiratoire et virus
por: Brouard, J, et al.
Publicado: (1999)

Single reaction, real time RT-PCR detection of all known avian and human metapneumoviruses
por: Lemaitre, E., et al.
Publicado: (2018)

Rapid identification viruses from nasal pharyngeal aspirates in acute viral respiratory infections by RT-PCR and electrospray ionization mass spectrometry()()
por: Chen, Kuan-Fu, et al.
Publicado: (2011)

Simultaneous detection of respiratory viruses in children with acute respiratory infection using two different multiplex reverse transcription-PCR assays
por: Wang, Wei, et al.
Publicado: (2009)

Cannot write session to /tmp/vufind_sessions/sess_9lk2k7g1ip6pkgese0dg8puj76