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Development of a novel real-time RT-PCR assay with LUX primer for the detection of swine transmissible gastroenteritis virus

Real-time RT-PCR assay, based on light upon extension (LUX) fluorogenic primer and LightCycle technology, was developed for rapid detection of transmissible gastroenteritis virus (TGEV). Viral RNA from different TGEV isolates and clinical specimens was detected. To evaluate the sensitivity of the as...

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Detalles Bibliográficos
Autores principales: Chen, Ru, Huang, Weiming, Lin, Zhixiong, Zhou, Zhongfang, Yu, Haiqiong, Zhu, Daozhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2004
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7119747/
https://www.ncbi.nlm.nih.gov/pubmed/15488621
http://dx.doi.org/10.1016/j.jviromet.2004.08.003