Cargando…
Effect of recombinant human interferon-alpha in vitro and in vivo on mitogen-induced lymphocyte blastogenesis in cats()
The effect of recombinant human interferon-alpha (rHuIFN-α) in vitro and in vivo on mitogen-induced lymphocyte blastogenesis was evaluated in specific-pathogen-free cats. Pre-incubation of isolated feline peripheral blood lymphocytes (PBL) in vitro with either 10(4) or 10(3) International Units (U)...
Autores principales: | , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Published by Elsevier B.V.
1990
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7119795/ https://www.ncbi.nlm.nih.gov/pubmed/2139993 http://dx.doi.org/10.1016/0165-2427(90)90017-M |
_version_ | 1783514836006600704 |
---|---|
author | Weiss, R.C. Oostrom-Ram, T. |
author_facet | Weiss, R.C. Oostrom-Ram, T. |
author_sort | Weiss, R.C. |
collection | PubMed |
description | The effect of recombinant human interferon-alpha (rHuIFN-α) in vitro and in vivo on mitogen-induced lymphocyte blastogenesis was evaluated in specific-pathogen-free cats. Pre-incubation of isolated feline peripheral blood lymphocytes (PBL) in vitro with either 10(4) or 10(3) International Units (U) of rHuIFN-α for 24 h significantly suppressed (P<0.001 and 0.01, respectively) blastogenic responses to the phytomitogens concanavalin A (Con A) and pokeweed mitogen (PWM). Lower doses of IFN (range, 10–10(−3) U/ml) neither suppressed nor enhanced mitogenesis. In the absence of phytomitogens, incubation of PBL with 10(4)–10(2) U (P<0.001) or 10 U (P<0.05) of rHuIFN-α/ml resulted in a significant decrease in incorporation of [methyl-(3)H] thymidine into newly synthesized cellular DNA. Cultures of PBL exposed continuously for 4 days to rHuIFN-α doses of 10(4) U/ml or less did not demonstrate specific reductions in cell viability, indicating that the observed antiproliferative actions of IFN apparently were independent of any direct cytotoxic effects. To investigate the dose-response effects of rHuIFN-α in vivo on lymphocyte blastogenesis, individual groups of cats were evaluated on 3 consecutive days before and then 24 h after each cat was inoculated intramuscularly with either a high dose (10(6) U/kg), moderate dose (10(4) U/kg), or a relatively low dose (10(2) U/kg) of rHuIFN-α. Cats inoculated with 10(6) U ofrHuIFN-α/kg had significantly reduced (P=0.037) blastogenic responses to Con a at 24 h postinoculation compared to preinoculation values; mean PWM responses were also decreased, but this effect was not statistically significant. In contrast, inoculation of cats with either 10(4) or 10(2) U of rHuIFN-α/kg significantly enhanced (P=0.05 or 0.008, respectively) Con A-induced blastogenesis and had no discernible effect on PWM responses. These findings suggest that very high doses of rHuIFN-α given parenterally may be associated with suppression of certain T-cell responses in cats; conversely, much lower doses may be immunoenhancing. |
format | Online Article Text |
id | pubmed-7119795 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1990 |
publisher | Published by Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71197952020-04-08 Effect of recombinant human interferon-alpha in vitro and in vivo on mitogen-induced lymphocyte blastogenesis in cats() Weiss, R.C. Oostrom-Ram, T. Vet Immunol Immunopathol Article The effect of recombinant human interferon-alpha (rHuIFN-α) in vitro and in vivo on mitogen-induced lymphocyte blastogenesis was evaluated in specific-pathogen-free cats. Pre-incubation of isolated feline peripheral blood lymphocytes (PBL) in vitro with either 10(4) or 10(3) International Units (U) of rHuIFN-α for 24 h significantly suppressed (P<0.001 and 0.01, respectively) blastogenic responses to the phytomitogens concanavalin A (Con A) and pokeweed mitogen (PWM). Lower doses of IFN (range, 10–10(−3) U/ml) neither suppressed nor enhanced mitogenesis. In the absence of phytomitogens, incubation of PBL with 10(4)–10(2) U (P<0.001) or 10 U (P<0.05) of rHuIFN-α/ml resulted in a significant decrease in incorporation of [methyl-(3)H] thymidine into newly synthesized cellular DNA. Cultures of PBL exposed continuously for 4 days to rHuIFN-α doses of 10(4) U/ml or less did not demonstrate specific reductions in cell viability, indicating that the observed antiproliferative actions of IFN apparently were independent of any direct cytotoxic effects. To investigate the dose-response effects of rHuIFN-α in vivo on lymphocyte blastogenesis, individual groups of cats were evaluated on 3 consecutive days before and then 24 h after each cat was inoculated intramuscularly with either a high dose (10(6) U/kg), moderate dose (10(4) U/kg), or a relatively low dose (10(2) U/kg) of rHuIFN-α. Cats inoculated with 10(6) U ofrHuIFN-α/kg had significantly reduced (P=0.037) blastogenic responses to Con a at 24 h postinoculation compared to preinoculation values; mean PWM responses were also decreased, but this effect was not statistically significant. In contrast, inoculation of cats with either 10(4) or 10(2) U of rHuIFN-α/kg significantly enhanced (P=0.05 or 0.008, respectively) Con A-induced blastogenesis and had no discernible effect on PWM responses. These findings suggest that very high doses of rHuIFN-α given parenterally may be associated with suppression of certain T-cell responses in cats; conversely, much lower doses may be immunoenhancing. Published by Elsevier B.V. 1990-02 2002-11-12 /pmc/articles/PMC7119795/ /pubmed/2139993 http://dx.doi.org/10.1016/0165-2427(90)90017-M Text en Copyright © 1990 Published by Elsevier B.V. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Weiss, R.C. Oostrom-Ram, T. Effect of recombinant human interferon-alpha in vitro and in vivo on mitogen-induced lymphocyte blastogenesis in cats() |
title | Effect of recombinant human interferon-alpha in vitro and in vivo on mitogen-induced lymphocyte blastogenesis in cats() |
title_full | Effect of recombinant human interferon-alpha in vitro and in vivo on mitogen-induced lymphocyte blastogenesis in cats() |
title_fullStr | Effect of recombinant human interferon-alpha in vitro and in vivo on mitogen-induced lymphocyte blastogenesis in cats() |
title_full_unstemmed | Effect of recombinant human interferon-alpha in vitro and in vivo on mitogen-induced lymphocyte blastogenesis in cats() |
title_short | Effect of recombinant human interferon-alpha in vitro and in vivo on mitogen-induced lymphocyte blastogenesis in cats() |
title_sort | effect of recombinant human interferon-alpha in vitro and in vivo on mitogen-induced lymphocyte blastogenesis in cats() |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7119795/ https://www.ncbi.nlm.nih.gov/pubmed/2139993 http://dx.doi.org/10.1016/0165-2427(90)90017-M |
work_keys_str_mv | AT weissrc effectofrecombinanthumaninterferonalphainvitroandinvivoonmitogeninducedlymphocyteblastogenesisincats AT oostromramt effectofrecombinanthumaninterferonalphainvitroandinvivoonmitogeninducedlymphocyteblastogenesisincats |