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Polymerase chain reaction for probe synthesis and for direct amplification in detection of bovine coronavirus
The polymerase chain reaction (PCR) was used to synthesize ds and ss probes for the detection of bovine coronavirus (BCV) using recombinant plasmids as template molecules. The ds probes detected a minimum of about 2 × 10(5) viral genomes after exposure for 1 h, a detection limit similar to nick-tran...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Published by Elsevier B.V.
1990
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7119836/ https://www.ncbi.nlm.nih.gov/pubmed/2266145 http://dx.doi.org/10.1016/0166-0934(90)90052-H |