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Detection of IgM responses to bovine respiratory syncytial virus by indirect ELISA following experimental infection and reinfection of calves: abolition of false positive and false negative results by pre-treatment of sera with protein-G agarose

The IgM responses in three panels of sera generated by infection and reinfection of calves with bovine respiratory syncytial virus (BRSV) were measured by indirect ELISA (I-ELISA). The effect of depleting serum IgG by pre-treatment with protein G agarose (PGA) was evaluated. Following primary infect...

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Detalles Bibliográficos
Autores principales: Graham, D.A., Foster, J.C., Mawhinney, K.A., Elvander, M., Adair, B.M., Merza, M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Science B.V. 1999
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7119899/
https://www.ncbi.nlm.nih.gov/pubmed/10522785
http://dx.doi.org/10.1016/S0165-2427(99)00086-0