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Detection of IgM responses to bovine respiratory syncytial virus by indirect ELISA following experimental infection and reinfection of calves: abolition of false positive and false negative results by pre-treatment of sera with protein-G agarose
The IgM responses in three panels of sera generated by infection and reinfection of calves with bovine respiratory syncytial virus (BRSV) were measured by indirect ELISA (I-ELISA). The effect of depleting serum IgG by pre-treatment with protein G agarose (PGA) was evaluated. Following primary infect...
Autores principales: | Graham, D.A., Foster, J.C., Mawhinney, K.A., Elvander, M., Adair, B.M., Merza, M. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier Science B.V.
1999
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7119899/ https://www.ncbi.nlm.nih.gov/pubmed/10522785 http://dx.doi.org/10.1016/S0165-2427(99)00086-0 |
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